For Laboratory Or Hospital High Accuracy Free Prostate-Specific Antigen Elisa Test Kit

High Accuracy Free Prostate-Specific Antigen Elisa Test Kit

INTENDED USE

The free PSA assay is an enzyme-linked immunosorbent assay (ELISA) for the in vitro quantitative determination of free prostate-specific antigen (fPSA) in human serum.

Test principle

• Sample, PSA-specific antibody coated microwells and enzyme labeled Anti-Free PSA monoclonal antibody are combined.

• During the incubation, Free PSA presents in the sample is allowed to react simultaneously with the two antibodies, resulting in the Free PSA molecules being sandwiched between the solid phase and enzyme-linked antibodies.

• After washing, a complex is generated between the solid phase, the Free PSA within the sample and enzyme-linked antibodies by immunological reactions.

• Substrate solution is then added and catalyzed by this complex, resulting in a chromogenic reaction. The resulting chromogenic reaction is measured as absorbance.

• The absorbance is proportional to the amount of Free PSA in the sample.

Materials provided

• Coated Microplate - symbol fPSA PLATE mouse monoclonal PSA-specific antibody.

• Calibrators - symbol fPSA PLATE 8 x 12 strips, 96 wells, pre-coated with 6 vials, 1 mL each, ready to use; Concentrations: 0(A), 1(B), 2.5(C), 5(D), 10(E) and 20(F) ng/mL.

• Enzyme Conjugate - symbol fPSA CONJ 1 vial, 11 mL of HRP (horseradish peroxidase) labeled Anti-Free PSA monoclonal antibody in Tris-NaCl buffer containing BSA (bovine serum albumin). Contains 0.1% ProClin300 preservative.

• Substrate - symbol TMB. SUBSTRATE STOP 1 vial, 11mL, ready to use, (tetramethylbenzidine) • Stop Solution - symbol 1 vial, 6.0 mL of 1 mol/L sulfuric acid.

• Wash Solution Concentrate - symbol PBS-Tween wash solution.

• IFU - 1 copy.

• Plate Lid: 1 piece.

• Plate Lid: 1 piece.

For Laboratory Or Hospital High Accuracy Free Prostate-Specific Antigen Elisa Test Kit Images