Vitamin B12 ELISA TEST KIT

Drug Names

Generic Name:VB12 ELISA Kit

Purpose

This kit allows for the determination of VB12 concentrations in sample.

Principle of the assay

The kit use ELISA competition to assay VB12 level in the sample,use Purified VB12 antibody to coat microtiter plate wells, make solid-phase antibody, add VB12 and antibody which With HRP labeled VB12 to coated microtiter wells,make them Combine competitive ,after washing Completely, Add TMB substrate solution . the depth of color and the VB12 of sample were positively correlated, measure the optical densit (OD) at 450 nm with microtiter plate reader, calculate VB12 concentration by standard curve.

Test principle

Materials provided with the kit

Important notes

• The kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature then use, ELISA plates coated if has not use up after opened, the plate should be stored in Sealed bag.
• washing buffer will Crystallization separation, it can be heated the water helps dissolve when

dilute . Washing does not affect the result.

• add Sample with sampler Each step, And proofread its accuracy frequently, avoids the experimental error. add sample within 5 min, if the number of sample is much , recommend to use Volley .
• Please make specification curve when you assay, had better make duplicate well, if in the sample the testing material content is excessively high (The sample OD is bigger than the first standard well OD ),please use Sample dilution to dilute certain multiple (n times),then assay. Please multiply total Dilution Times when calculate(×n×5).
• Closure plate membrane only limits the disposable use, in order to avoid the overlapping pollution.
• The substrate please evade the light preservation.
• Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard .
• All samples, washing buffer and each kind of reject should according to infective material process.
• This reagent which different batch number component do not mix.
• If it’s different form English instruction, take English instruction as the standard.

Storage and stability

• Store at 2-8℃.

• Seal and return unused reagents to 2-8℃, under which conditions the stability will be retained for 2 months, or until the labeled expiry date, whichever is earlier.

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