Bovine Brucellosis
FOR RESEARCH USE ONLY
Drug Names
Bovine Brucellosis elisa test kit RUO
Purpose
This kit allows for the determination of Brucellosis expression in Bovine serum, and other biological fluids.
Principle of the assay
The kit assay Brucellosis level in the sample, use Purified Brucellosis antibody to coat microtiter plate wells, make solid-phase antibody, then add Brucellosis to wells, Combined With Brucellosis, after washing and removing non-combinative antigen and other components ,then Combined Brucellosis antibody which with HRP labeled become antibody – antigen - enzyme- antibody complex, after washing Completely, Add TMB substrate solution,, TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. Compared with the CUTOFF value, according to this to judge Brucellosis exist in the sample or not.
| Product details | Description |
| Delivery | Within 48 hours |
| Packaging Specifications | 8 x 12 strips, 96 wells |
| Country Of Origin | China |
| Manufacturer | 18 months |
| Preservation method | 2℃-8℃ |
| Specimen | Whole blood |
| Assification | class1 |
| Type | Elisa Test Kit |
Test principle
Materials provided with the kit
| 1 | wash solution | 20ml×1bottle | 8 | 1 Standard(48nmol/L) | 0.5ml×1 bottle |
| 2 | HRP-Conjugate reagent | 6ml×1 bottle | 2 Standard(24nmol/L ) | 0.5ml×1 bottle |
| 3 | Microelisa stripplate | 12well×8strips | 3 Standard(12nmol/L) | 0.5ml×1 bottle |
| 4 | Stop Solution | 6ml×1 bottle | 4 Standard(6nmol/L) | 0.5ml×1 bottle |
| 5 | Chromogen Solution A | 6ml×1 bottle | 5 Standard(3nmol/L) | 0.5ml×1 bottle |
| 6 | Chromogen Solution B | 6ml×1 bottle | 9 | User manual | 1 |
| 7 | Sample diluent | 6ml×1 bottle | 10 | Closure plate membrane | 2 |
Important notes
Specimen requirements Sealed bags
1. extract as soon as possible after Specimen collection,and according to the relevant literature, and should be experiment as soon as possible after the extraction. If it can’t, specimen can be kept in -20 ℃ to preserve, Avoid repeated freeze-thaw cycles.
2. Can’t detect the sample which contain NaN3, because NaN3 inhibits HRP active.
Storage and stability
• Store at 2-8℃.
• Seal and return unused reagents to 2-8℃, under which conditions the stability will be retained for 2 months, or until the labeled expiry date, whichever is earlier.
Product Tags:
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Brucella elisa test kit RUO Elisa Test Kit Of Enzyme-Linked Product RESEARCH USE ONLY Animal Test Images
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