TOXO IgG ELISA Test Kit

Intended use

Toxo IgG Elisa test is intended for the detection and quantitative determination of IgG antibody to TOXO in human sera.

ASSAY PROCEDURE

1. Prepare Reagents: Dilute 1 volume of Washing Buffer (20×)with 19 volumes of distilled water, mix well.

2. Add Samples: Open the foil pouch and remove the Microplate. Set up 1 well as Blank, 2 wells as negative control, 2 wells as positive control. After dispensing 100μL of Sample Diluent , dispense 10μL of sample, 100μL negative control or positive control to the respective wells(without Sample Diluent). Gently vibrating the plate.

3. Incubate: Cover the Microplate with plate cover and incubate the Microplate in a thermostat-controlled water-bath or microplate incubator at 37℃ for 30 minutes.

4. Wash the Plate: Remove the plate cover. Aspirate the contents of all wells. Fill the wells with the diluted washing buffer ( 10~20 seconds to soak) then aspirate again. Repeat the procedure for 5 times. Make sure that the rest volume is minimal, by tapping plate onto absorbent paper.

5. Add Conjugate: Add 100μL of conjugate to each well (except the blank well).

6. Incubate: Cover the Microplate and incubate the plate at 37℃ for 30 minutes.

7. Wash the Plate: Repeat the wash procedure as in step 4.

8. Add Substrate: Add 50μL of Substrate Solution A and 50μL of Substrate Solution B to each well, mix well. Cover and incubate at 37℃ for 15 minutes.

9. Stop reaction: Add 50μL Stop Solution to each well, mix well.

10. Read the absorbance at 450 nm. If a dual wavelength measurement is used, the reference wavelength should be selected from 620nm to 690nm.

RESULTS

1. For the assay to be valid, the mean OD value of negative controls must be less than or equal to 0.1 and the mean OD value of positive controls must be greater than or equal to 0.8. 2. Cut off value = NCx2.1 NC= the mean absorbance value for two negative controls When NC is less than 0.05, please calculate as 0.05. 3. OD value of the sample  cut off value, it is positive for anti-Toxo IgM. OD value of the sample  cut off value, it is negative for anti-Toxo IgM. PRECAUTIONS 1. Allow all kit components to reach room temperature before use. 2. Follow the direction insert to control the reaction temperature and time strictly.

3. Do not mix components of different lot numbers to use.

4. The kit should be store at 2-8℃. Do not use kit components beyond their expiration date.

Technical Parameters:

Specimen collection and preparation

• Human serum or plasma is recommended for this assay.

• Cap and store the samples at 18-25 ℃ for no more than 8 hours. Stable for 7 days at 2-8℃, and 1 month at -20℃. Freeze only once.

• Do not use heat-inactivated samples.

• Sediments and suspended solids in samples may interfere with the test result which should be removed by centrifugation. Ensure that complete clot formation in serum samples has taken place prior to centrifugation.

• Avoid grossly hemolytic, lipemic or turbid samples.

FAQ:

Q: What is the brand name of this test kit?

A: The brand name of this test kit is Biovantion.

Q: Where is this test kit manufactured?

A: This test kit is manufactured in China.

Q: Is this test kit certified?

A: Yes, this test kit is certified with ISO13485.

Q: What is the minimum order quantity for this product?

A: The minimum order quantity for this product is 10.

Q: What is the packaging details for this product?

A: The packaging details for this product is CARTON.

Q: What is the delivery time for this product?

A: The delivery time for this product is 7-15 days.

Q: What are the payment terms for this product?

A: The payment terms for this product is TT 100% PAYMENT.

Q: What is the supply ability for this product?

A: The supply ability for this product is 100000.

Q: Is the price of this product negotiable?

A: Yes, the price of this product is negotiable.