Ferritin Elisa Test Kit

INTENDED USE

The Ferritin assay is an enzyme-linked immunosorbent assay (ELISA for the in vitro quantitative determination of ferritin in human serum. This assay is useful in diagnosis and screening of anemia. For professional use only.

Summary

Ferritin is a macromolecule with a molecular weight of at least 440 kD (depending on the iron content) and consists of a protein shell (apoferritin) of 24 subunits and an iron core containing an average of approx. 2500 Fe3+ ions (in liver and spleen ferritin).1 In humans, it acts as a buffer against iron deficiency and iron overload.2 If the ferritin level is low, there is a risk for lack of iron, which could lead to anemia or iron deficiency without anemia. In the setting of anemia, low serum ferritin is the most specific lab finding for iron-deficiency anemia.3 Low ferritin may also indicate hypothyroidism, vitamin C deficiency or celiac disease. Low serum ferritin levels are seen in some patients with restless legs syndrome, not necessarily related to anemia, but perhaps due to low iron stores short of anemia.4,5 A normal ferritin blood level, referred to as the reference interval is determined by many testing laboratories. The ranges for ferritin can vary between laboratories but typical ranges would be between 30–300 ng/mL (=µg/L) for males, and 30–160 ng/mL (=µg/L) for females. A value less than 50 is considered as iron deficiency. Serum ferritin levels are measured in medical laboratories as part of the iron studies workup for iron-deficiency anemia.6 The ferritin levels measured usually have a direct correlation with the total amount of iron stored in the body.

Test principle

Sandwich principle. Total duration of assay: 80 minutes.
• Sample, Anti-Ferritin coated microwells and enzyme labeled Anti-Ferritin are combined.
• During the incubation, ferritin presents in the sample is allowed to react simultaneously with the two antibodies, resulting in the ferritin molecules being sandwiched between the solid phase and enzyme-linked antibodies.
• After washing, a complex is generated between the solid phase, the Ferritin within the sample and enzyme-linked antibodies by immunological reactions.
• Substrate solution is then added and catalyzed by this complex, resulting in a chromogenic reaction. The resulting chromogenic reaction is measured as absorbance.
• The absorbance is proportional to the amount of ferritin in the sample.


Technical Parameters:

Specimen collection and preparation

• Human serum is recommended for this assay.
• Cap and store the samples at 18-25 ℃ for no more than 8 hours. Stable for 7 days at 2-8℃, and 1 month at -20℃. Freeze only once.
• Do not use heat-inactivated samples.
• Sediments and suspended solids in samples may interfere with the test result which should be removed by centrifugation. Ensure that complete clot formation in serum samples has taken place prior to centrifugation.
• Avoid grossly hemolytic, lipemic or turbid samples.

FAQ:

Q: What is the brand name of this test kit?
A: The brand name of this test kit is Biovantion.
Q: Where is this test kit manufactured?
A: This test kit is manufactured in China.
Q: Is this test kit certified?
A: Yes, this test kit is certified with ISO13485.
Q: What is the minimum order quantity for this product?
A: The minimum order quantity for this product is 10.
Q: What is the packaging details for this product?
A: The packaging details for this product is CARTON.
Q: What is the delivery time for this product?
A: The delivery time for this product is 7-15 days.
Q: What are the payment terms for this product?
A: The payment terms for this product is TT 100% PAYMENT.
Q: What is the supply ability for this product?
A: The supply ability for this product is 100000.
Q: Is the price of this product negotiable?
A: Yes, the price of this product is negotiable.