Product Description:
FSH (follicle stimulating hormone), together with LH (luteinizing hormone),belongs to the gonadotropin family. FSH and LH regulate and stimulate the growth and function of the gonads (ovaries and testes) synergistically. Like LH, TSH and hCG, FSH is a glycoprotein consisting of two subunits (α- and β‐chains). Its molecular weight is approx. 32000 daltons. In women, the gonadotropins act within the hypothalamus‐pituitary‐ovary regulating circuit to control the menstrual cycle. (1, 2, 3) FSH and LH are released in pulses from the gonadotropic cells of the anterior pituitary. The levels of the circulating hormones are controlled by ster0id hormones via negative feedback to the hypothalamus. In the ovaries FSH, together with LH, stimulates the growth and maturation of the follicle and hence also the biosynthesis of estr0gens in the follicles. The FSH level shows a peak at mid-cycle, although this is less marked than with LH. Due to changes in ovarian function and reduced estr0gen secretion, high FSH concentrations occur during menopause. (3) In men, FSH serves to induce spermatogonium development. Determination of the FSH concentration is used in the elucidation of dysfunctions within the hypothalamus‐pituitary‐gonads system. The determination of FSH in conjunction with LH is utilized for the following indications: congenital diseases with chromosome aberrations, polycystic ovaries (PCO), amenorrhea (causes), and menopausal syndrome. Depressed gonadotropin levels in men occur in azoospermia. (1, 2, 3, 4, 5)
Test Principle:
Sandwich principle. Total duration of assay: 80 minutes.
• Sample, Anti-FSH coated microwells and enzyme labeled Anti-FSH are combined.
• During the incubation, FSH presents in the sample is allowed to react simultaneously with the two antibodies, resulting in the FSH molecules being sandwiched between the solid phase and enzyme-linked antibodies.
• After washing, a complex is generated between the solid phase, the FSH within the sample and enzyme-linked antibodies by immunological reactions.
• Substrate solutionis then added and catalyzed by this complex, resulting in a chromogenic reaction. The resulting chromogenic reaction is measured as absorbance.
• The absorbance is proportional to the amount of FSH in the sample.
Technical Parameters:
| Product Name | FSH Elisa Test |
| Manufacturer | Biovantion |
| Test Type | ELISA |
| Format | Kit |
| Specimen | Serum 50μl |
| Sensitivity | High |
| Specificity | High |
| Applications | Hormones Diagnosis |
| Assay Time | 1 Hour |
| Shelf Life | 18 Months |
Materials provided:
• Coated Microplate, 8 x 12 strips, 96 wells, pre-coated with mouse monoclonal Anti-FSH.
• Calibrators, 6 vials, 1 ml each, ready to use; Concentrations: 0(A), 5(B), 20(C), 50(D), 100(E) and 200(F) mIU/mL.
• Enzyme Conjugate, 1 vial, 11 mL of HRP(horseradish peroxidase) labeled mouse monoclonal Anti-FSH in Tris-NaCl buffer containing BSA (bovine serum albumin). Contains 0.1% ProClin300 preservative.
• Substrate, 1 vial, 11ml, ready to use, (tetramethylbenzidine) TMB.
• Stop Solution, 1 vial, 6.0 ml of 1 mol/l sulfuric acid.
• Wash Solution Concentrate, 1 vial, 25 ml (40X concentrated), PBS-Tween wash solution.
• IFU, 1 copy.
• Plate Lid: 1 piece.
Storage:
• Store at 2-8℃.
• Place unused wells in the zip-lock aluminum foiled pouch and return to 2-8 °C, under which conditions the wells will remain stable for 2 months, or until the labeled expiry date, whichever is earlier.
• Seal and return unused calibrators to 2-8 °C, under which conditions the stability will be retained for 1 month, for longer use, store opened calibrators in aliquots and freeze at -20 °C. Avoid multiple freeze-thaw cycles.
• Seal and return all the other unused reagents to 2-8 °C, under which conditions the stability will be retained for 2 months, or until the labeled expiry date, whichever is earlier.
