Estradial E2 ELISA Test Kit

Drug Names

Generic Name:Estradial E2 ELISA Test Kit

Purpose

For in vitro diagnostic and professional use only.

SUMMARYANDCLINICALSIGNIFICANCE

Estradiol (E2, oestradiol) is a sex hormone. Estradiol is abbreviated E2 as it has two hydroxylgroups in its molecular structure. Estradiol is about 10 times as potent as estrone and about 80 times as potent as estriol in its effect. Except during the early follicular phase of the menstrual cycle, its serum levels are somewhat higher than that of estrone during the reproductive years of the human female. Thus it is the predominant during reproductive years both in terms of absolute serum levels as well as in terms of activity. During menopause, estrone is the predominant circulating and during pregnancy estriol is the predominant circulating in terms of serum levels. Serum estradiol levels have clinical valuable of diagnosis menstrual abnormalities, menopause, infertility and precocious puberty

PRINCIPLE

E2 kit is based on competitive method. Coat micro well plates with E2 antibody. Add E2 calibrator (or patient sample) and HRP-E2 to form E2-antibody—HRP-E2 complex. The binding amount of HRP-E2 is reversely proportional to E2 content in serum. Remove the unbound E2-HRP. Add substrate and detect absorbent value. Calculate E2 content of patient serum through computer or plotting fitting concentration-absorbent value curve.

Important notes

• The kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature then use, ELISA plates coated if has not use up after opened, the plate should be stored in Sealed bag.
• washing buffer will Crystallization separation, it can be heated the water helps dissolve when

dilute . Washing does not affect the result.

• add Sample with sampler Each step, And proofread its accuracy frequently, avoids the experimental error. add sample within 5 min, if the number of sample is much , recommend to use Volley .
• Please make specification curve when you assay, had better make duplicate well, if in the sample the testing material content is excessively high (The sample OD is bigger than the first standard well OD ),please use Sample dilution to dilute certain multiple (n times),then assay. Please multiply total Dilution Times when calculate(×n×5).
• Closure plate membrane only limits the disposable use, in order to avoid the overlapping pollution.
• The substrate please evade the light preservation.
• Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard .
• All samples, washing buffer and each kind of reject should according to infective material process.
• This reagent which different batch number component do not mix.
• If it’s different form English instruction, take English instruction as the standard.

Storage and stability

• Store at 2-8℃.

• Seal and return unused reagents to 2-8℃, under which conditions the stability will be retained for 2 months, or until the labeled expiry date, whichever is earlier.

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Estradial E2 ELISA Test Kit

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